Many studies indicated that Costus genus plant exhibited high bioactivities such as anticancer, apoptotic effect, anti-inflammatory, antidiabetics, antifungal, and antibacterial properties which revealed the potential values of these plants for nutraceutical application. This study proposed the use of sequential extraction with solvents of different polarities to elute maximum amount of compounds present in Costus woodsonii plant. Sequential extraction procedures have shown a rapid increase in applications, in a wide range of sample types. woodsonii plant and result indicated high polarity solvent (methanol and water) recovered higher bioactive compounds. In short, sequential extraction provides understanding of polarity specificity of C. Overall, methanolic extracts showed the significantly ( p ≤ .05) highest ascorbic acid content (75.76 ± 1.47 mg/100 ml), total phenolic content (249.64 ± 2.83 mg GAE/100 g), DPPH free radical-scavenging activity (78.76 ± 0.97%), ferric reducing antioxidant power (22.55 ± 0.96 mg TEAC/g), superoxide anion scavenging activity (46.60 ± 1.57%) and hydroxyl radical-scavenging activity (50.35 ± 0.96%).
Phytochemical screening showed positive presence of flavonoids (all types of extracts), terpenoids (only in methanolic extract), and tannins (except aqueous extract). woodsonii bract, at increasing polarities. Solvents of different polarities (hexane, ethyl acetate, methanol, and water) were used to sequentially extract the C. The current study reports the composition, phytochemical, and antioxidant properties of red button ginger ( Costus woodsonii) using sequential extraction. Costus plant is considered as a medicinal plant especially in therapeutic intervention.
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